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FLOWLOGIC SOFTWARE will be at: 53rd ASI Annual

Scientific Meeting – Perth, 1–5 December 2025

WEBINAR: Flow Analysis Guide 18 March 2025

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FlowLogic

• FlowLogic intro

• FlowLogic Webinar

• WEBINAR: Flow Analysis Guide 18 March 2025

• Webinar-FlowlogicSolution 1.2 Quick View

FlowLogic Solution 1.3:

• Auto-Compensation-Check-And-Adjustment

• Clustering-Phenotyping-Gating-Graphing

• Experiment-Template

• Folder-Template

• 25 Colors-Comp-Check

• 25 Colors-Clustering-Phenotyping

• High-throughput screening

FlowLogic Solution 1.2:

• PlateLogic: new layout and functionality

• Overlay: Side settings panel and Merge settings

• ClusterLogic: tree display and horizontal display with axes

• Contour quality improved check all quality display

• Compensation: remove -H and -W Parameters

• Export data with index and without index

• Mac native popup for save and load and export

Flowlogic Solution 1.1:

• Open your FlowJo WSP experiment  (*.WSP)

• Export FCS with updated compensation matrix

• Comp-Check: Auto and Finish

• Updated Auto-Titration: Auto-Titration

• Parameter setting for Display and Export

• Parameter compression auto settings at 3 diff level and manual settings

• On plots sliders to adjust x and y compression

• On scaling side panel, change Dot Size

• Updated Main window with Open Selected button

• Updated Overlay: double-click on a row in the table

• QCLogic: sorted keywords, added Fluorescence

• Reporting action on bottom left of main window

• Auto-compensation: Auto-Gate Positions improved

• New Auto-Gate

• Update ClusterLogic Globa display

• Only one Flowlogic can start

FlowLogic Solution 1.0:

• Quick Access

• QCLogic

• Parameters and Bi-Exp Display

• Compensation Check and Fix

• Compare Gates

• ClusterLogic Summary

• ClusterLogic Compare

• ClusterLogic Global

• ClusterLogic Fingerprint

• ClusterLogic Manual Clustering and Phenotype

• ClusterLogic Semi-Auto Clustering and Phenotype

• Auto-Voltration

• Advance Proliferation

PlateLogic Analysis

• Plate Functionality

• Plate Analysis

• Plate Overlay

• Plate GraphLogic

• Plate Analysis CD4 CD8

• PlateLogic: Heatmaps, Tags and Filtering 

• Building Plates from the File Navigator 

• Importing Plate Data into GraphLogic 

CompLogic Auto-Compensation

• CompLogic Tutorial 1 – Auto-compensation

• CompLogic Tutorial 2 – Auto-compensationwith a universal negative

• CompLogic Tutorial 3 – Spread Quantification Index (SQI) in CompLogic

FlowLogic Tips & Tricks

• Pasting gates to multiple files quickly and easily

• Jump, batch, pin and hide – tools in the File Navigator

• Launch FlowLogic from the command line

FlowLogic Licences

• Your online account overview

• Account registration, free trials and adding licence codes

• Managing the FlowLogic floating licence

• Sharing a floating licence

Analysis Basics

• Program Preferences

• Importing Files

• The File Navigator

• Creating and Managing Gates

• The Plot Side Drawer

• The Gate List Tab

• Compensation

• Displaying Multiple Statistics on Plots

• Saving Your Analysis

Advanced Features

• Overlays

• Group Analysis

• Statistics – Generating, Viewing and Exporting

• Boolean Gates

• Auto-compensation 

• Axis Range Adjustment

• GraphLogic

• Graphing Two Independent Variables 

• Two-way ANOVA

• DocLogic

• Inserting Statistics Tables in DocLogic 

• TitrateLogic

• File Replacement

• Global Analysis Templates 

• Merging Multiple Analysis Files – Open Append

• IndexLogic 

• MQD Volumetric Statistics 

• Splitting Grouped MQD Files

• Cell Cycle Analysis 

• Proliferation Analysis

• Importing Keywords from CSV Files

• Folder Actions

• BeadLogic Setup in FlowLogic 

• BeadLogic Analysis Workflow